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After tipping off the supernatant, diluted supernatants were incubated for 2 hr at RT

The three most frequently reported PTs were cataract (n = 78), macular degeneration (n = 42), and glaucoma (n = 33)

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The resulting RNA-VQ-TGIRT complex was parameterized and output as Amber prmtop and inpcrd files, as well as a PDB reference file, for downstream MD simulations